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CALNEXIN

As with calcineurin, an example of copy being prepared for the 2nd editon of the Biochemistry and Molecular Biology Compendium. Commetns and corrections will be appreciated.

CALCINEURIN

Another example of the material being revised for the second edition of the Bochemistry and Molecular Biology Compendium

ANTISENSE PEPTDIES - WHAT A DECADE DOES! ALSO KNOWN AS COMPLEMENTARY PEPTIDES

I continue to be intrigued by antisense peptides - also known as complementary peptides -There is quite difference between te 2007 entry and the 2017 entiry.

 

 

Antibody Entry for Compendium Revision

The Compendium for Biochemistry and Molecular Biology is being revised. I am including the antibody entry to provide an illustration of the extend of revision.

 

 

FAKE RESEARCH REAGENTS

The issue of fake research reagents is an increasing problem, The problem is of great concern with antibody reagents.

 

 

Second Edition of Biochemistry and Molecular Biology Compendium is in development

 

GLYCOPEGYLATION AND FACTOR VIII

The quest for a factor VIII with extended half-life goes on.

 

STABILITY OF ANTIBODY-DRUG CONJUGATES

Maleimide chemistry just gets more interesting and useful - Some thoughts from work intended for the 5th Edtion of the Handbook of Biochemistry and Molecular Biology

 

 

MICHAEL ADDITIONS WITH FUMARATE AS MICHAEL DONOR

I came across some old studies on the formation of conjugates between fumarate and thiols and was interested to see some interesting studies subsequent to the early observations by Baynes and coworkers.

 

 

A NOVEL ASSAY FOR PHOSPHOLIPASE A

A novel assay using a reagent obtained at local retail

MIT Technology Review has an interesting article on gene therapy in the November/December, 2016 is (p.25) - Entitled "Gene Therapy Cure has Money-Back Guarantee."

 

FACTOR VIII GENE THERAPY NOVEMBER 2016

Convalescent Serum and Convalesent Plasma and Hyperimmune Globulins

Convalescent plasma is being used in the current Ebola crisis - the use of these products dates to 1918.

CONSIDERATIONS OF ADVANCES IN HEMOPHILIA THERAPEUTICS

 

 

BUS RIDING AND READING - Roast Pig, Lutefisk and hydroxycitrate

 

Some Interesting Articles on Gene Therapy

Ex vivo use of stem cells is moving quickly

 

Some MoreThoughts on Factor VIII Mimetics

Is there anything that truly substitute for factor VIII function?

 

 

Ion-Exchange Resins, Blood and Factor IX with a Note on Serum as Therapeutic

Zymogenicity

 

THROMBIN ADSORPTION TO FIBRIN

Some thoughts on the interaction of thrombin with fibrin including the affinity purification of thrombin in 1845.

PROTHROMBIN COMPLEX CONCENTRATES AND FACTOR VIIa

A recent article on thrombosis following use of prothrombin complex concentrates (Factor IX concentrates) and factor VIIa took me back to work in my laboratory in Chapel Hill some 40 years ago.

 

Arrowsmith, Clinical Trials, Compassionate Use and Phosphethanolamine

Sinclair Lewis has a message for today's scientific community

 

 

BLOODCURDLING MOVIES AND FACTOR VIII

Another interesting contribution to the lore of Factor VIII from Nemeth and coworkers in Leiden

 

Kidney organoids from stem cells

Future of organ tranplatation and a new platform for drug testing.

 

Gene-Editing takes Gene Therapy into Warp Drive

The future is now for gene therapy!

 

ANOTHER USE FOR FIBRIN SEALANT - FOOD PROCESSING

A description of the use of fibrin sealant in food processing.

 

Factor VIII in Interstitial Space

A discussion of the presence of factor VIII in the interstitial space and a possible role in fibrosis.

LUNG TRANSPLANTATION CURE VON WIILLEBRAND DISEASE

 

Some old observations and a Nature editorial

Why are some papers just missed?

 

HYALURONAN AND FIBRINOGEN - A UNIQUE COMBINATION

There is a unique interaction of hylauronan with fibrinogen that is likely of importance within the interstitial space,

 

A new (old) book on sulfhydryl chemistry

Jocelyn, P.C., Biochemistry of the SH Group, Academic Press, London, United Kingdom, 1972

 

CHO PROTEIN IN A THERAPETIC MAB

This is a very good article on the characterization of a protein impurity present in a monoclonal antibody therapeutic under development. It is an example how. despite my complaint of below,there is good science in biotechnology today.

CYSTEINE IN FACTOR VIII - 2015 UPDATE

 

CURRENT STATUS OF THE BIOMEDICAL RESEARCH ENTERPRISE (OR LACK THEREOF)

 

The Reaction of Ethylene Oxide with Biopharmaceuticals in Delivery Devices - Old Chemistry with new Analytical Technology

 

Gamma-thrombin for anticoagulant neutralization and thrombin for pain control - Thrombin goes to new places

 

 

Antibiotics from Soil - A Blast from the Past

Convalescent Serum is Passive Immunization in Ebola

 

 

Hazards in the Use of Synthetic Substrates for Recombinant Streptokinase

 

 

DISULFIRAM (Antabuse)

Some interesting material on the history of the development of antabus

Recent Developments in Therapeutics for Hemophilia A

Emphasis placed on the development of Factor VIII products with extended circulatory half-lives.

FACTOR VIII MIMICS IN THE TREATMENT OF HEMOPHILIA A-2013

A consideration of the development of mimics of factor VIII for use in the treatment of hemophilia A.

Ortholog Non-Equivalence in Matrix Metalloproteinase Function.

How far can you trust mouse data?

 

Thrombin versus Fibrin Sealant: Flathead versus Phillips

 

BIOMARKER DISCOVERY AND EVALUATION

 

Glandular Kallikrein in Saliva and Cancer

 

ORAL PROTEIN DRUG DELIVERY

 

Chemical Reagents for Protein Modification, 4th edition.

This is a major revision of the 3rd edition which was published in 2005. It is being published by CRC Press/Taylor and Francis).(http://www.crcpress.com)

 

Substitutes for Factor VIII in Hemophilia

A brief history of the development of mimeticss for Factor VIII which might have potential for therapeutic use.

 

Standard Operating Procedures and

Normalization of Deviance

A short comment on the importance of standard operating procedures for corporate survival. Adherence to standard operating proedures are critical to avoid normalization of deviance.

 

Evolution of Therapeutics for Hemophilia A

This is historical perspective on the development of therapeutics for hemophilia A. Most recent developments are posted above

An excellent book for those in the plasma biz.

Blood, Plasma and Plasma Proteins. A Unique Contribution to Modern Healthcare, ed. J.L. Valverde, IOS Press, Amsterdam, Netherlands, 2006

Cysteine Sulfenic Acid

Cysteine sulfenic acid is is of increasing interest and may be more common than expected. While revising Chemical Reagents for Protein Modification I have noticed a possible relationship to cystine oxides which may be of importance. I have also noticed that cysteine sulfenic acid can react with reagents such as N-ethylmaleimide.

 

IGIV and Infectious Disease -An under-apprecited application of an important biopharmaceutical product.

 

Serum is not Plasma is not Whole Blood

 

C1-Esterase Inhibitor -a serpin with multiple functions

 

Implants and Infection

I have recently celebrated the three month anniversary of my right knee athroplasty. Having time on my hands, I have gone back to topics from the past. One of these concerned the topical use of intravenous immunoglobulin and infection of implants. Fortunately I have avoided infection and am looking forward to spring practice where I might have an opportunity as a tackling dummy.

 

Hyperimmune Globulins

This post is being written on March 13, 2012 (Friday the 13tth comes on a Tuesday this month). The Ides of March are on the 15th and Saint Patrick's Day is this Saturday - thus green for the title. My recent total knee arthroplasty got me thinking about infecetious disease again and the potential of hyperimmune globulins - which encouraged me to assemble some thoughts on the matter. Given the issues of antibiotic resistance and emerging pathogens, passive immunization becomes an attractive consideration.

Salivary Kallikrein and Cancer

Many years ago I and colleagues observed that glandular kallikrein was elevated in saliva from patients with solid tumors distant from the oral cavity. This is still an observation looking for a explanation.

DETERMINATION OF PROTEIN CONCENTRATION

The days of concern about specific activity of proteins appears to vanished except for those involved in biopharmaceutical manufacture - here this analysis is an important part of product characterization. Sapan and Lundblad have a new contribution to methods for determination of protein concentration: Sapan, C.V. and Lundblad, R.L. (2015), Review of methods for determination of total protein concentration and peptide concentration in biological samples, Proteomics Clinical Applications 9, 268-276

 

ALLERGOIDS

Spring has come to the Research Triangle and the first pollen alert of the year was promulgated by the Weather Service today (February 28, 2012). I had lived in Chapel Hill for some 10 years before the infamous Chapel Hill pollen struck and has remained a blot on my existance ever since; going north to the Federation meetings in Atlantic City was a considerble relief. I have recently become award of allergoids and wish that some enterprising company interested in a project in practical immunotherapy would take note.

PLASMA PROTEINS IN THE EXTRAVASCULAR SPACE - I was surprised (modest understatment) on the amount of plasma protein outside of the vascular space. Fully 60% of albumin is outside of vascular space and the values for other plasma protein vary from 1% to 70%. While the rate of extravasation may depend on molecular size but this is not hard consideration. Some initial thoughts have been collected. Further material will appear in the future. At the moment the author is somewhat handicapped recovering from total knee replacement (result of a misspent youth and an oblate spheroid). This is an ongoing effort with new material being added on a sporadic basis. I was somewhat surprised (and embarrassed that I was surprised) to learn about the importance interstitial protein in transfusion medicine.

Plasma Proteinase Inhibitors This is taken from the end of a chapter from a book on biotechnology of plasma proteins

Serpins - More that Protease Inhibitors

Some speculations on antithrombin Some thougths on the requirements for high doses of antithrombin for acquired antithrombin deficiency

Some Thoughts on Innovation.

The group in the Department of Physical Chemistry at Harvard Medical School made many discoveries during their work on blood plasma fractionation during World War II. One of the somewhat overlooked projects was that of thrombin and fibrin foam. This product has seen a bit of renaissance but the illustreous background is somewhat ignored.

History of the delivery of thrombin to the gastrointestinal tract

A brief consideration of the history of plasma fractionation as well as some thoughts on the future.

Back to the Future or Play it Again Sam - some ruminations on how enzymology changes and remains the same.

Factor VIII Past Present and Future

Oral Protein Drug Delivery and Hemophilia A

In Vitro Assays for Therapeutic Enzymes There has been considerable interest in the use of enzyme kinetic analysis in the characterization of therapeutic enzymes. Some considerations are presented for discussion.

Factor VIII - Reducing Agents, Copper Ions, and Stability Some speculation on free sulfhydryl groups in factor VIII, copper ion and solution stability.

Use of Thrombin for the Treatment of Esophageal Varices

Update on the Therapeutic use of Thrombin