Targeted Proteomics


The original definition of targeted proteomics was the analysis of a defined portion of a proteome such as a glycoproteome, phosphoproteome, ribosomal proteins, or a specific tissue proteome.(1-5) The concept of target proteomics has now been modified to describe procedures which analyze a narrow range of product ions using techniques such as selected reaction monitoring/multiple reaction monitoring to measure a specific protein.(6-10).

1.  Knepper, M.A. and Masilamani, S., Targeted proteomics in the kidney using ensembles of antibodies, Acta Physiol.Scand. 173, 11-21, 2001.
2.  Warcheid, B. and Fenselau, C., A targeted proteomics approach to the rapid identification of bacterial cell mixtures by matrix-assisted laser desorption/ionization mass spectrometry, Proteomics 4, 2877-2892, 2004.
3.  Mirzaei, H. and Regnier, F., Structure specific chromatographic selection in targeted proteomics, J.Chromatog.B Analyt.Technol.Biomed.Life.Sci. 817, 23-34, 2005.
4. Freije, J.F. and Bischoff, R., The use of affinity sorbents in targeted proteomics, Drug.Discov.Today Technol. 3. 5-11, 2006.
5. Portelius, E., Zetterberg, H., Gobom, J., Andreasson, U., and Blennow, K., Targeted proteomics in Alzheimer’s disease: focus on amyloid-β, Expert Rev.Proteomics 5, 225-237, 2008.à
6. Bereman, M.S., MacLean, B., Tomazela, D.M., Liebler, D.C., and MacCoss, M.J., The development of selàected reaction monitoring methods for targeted proteomics via empirical refinement, Proteomics 12, 1134-1141, 2012.
7.  Ebhardt, H.A., Root, A., Sander, C., and Aebersold, R., Applications of targeted proteomics in systems biology and translational medicine, Proteomics 15, 3193-3208, 2015.
8.  Shi, T., Song, E., Nie, S., et al., Advances in targeted proteomics and applications to biomedical research, Proteomics 16, 2160-2182, 2016.
9.  Borràs, E. and Sabidó, E., What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry, Proteomics 17:1700180, 2017.
10. Arsova, B., Watt, M., and Usadel, B., Monitoring of plant protein post-translational modifications using targeted  proteomics, Front.Plant Sci. 9:1168, 2018