Issues with the Therapeutic Use of DNAzymes

Victor, J.,Steger, G., and Riesner,D., Inability of DNAzymes to cleave RNA in vivo is due to limited Mg2+ concentration in cells, Eur.Biophys.J. in press (doi:10.1007/s00249-017-1270-2), 2018

I am working on a second edition of the Biochemistry and Molecular Biology Compendium and a lot has happened since 2006 when I wrote the first edition.  I have also come across some unexpected material. One such is the observation that while a DNAzyme was effect in “silencing” a message in vitro, the in vivo results did not show the same effect,  The authors astutely observed the  intracellular concentration of magnesium ions was not high enough to support the activity of the DNAzyme.