What a difference a decade makes! This 2017 version is for the second edtion of the Biochemistry and Molecular Biology Compendium

2007 Version
Antisense Peptides
The products from the translation of antisense RNA. Some antisense peptides have been demonstrated to show affinity properties that appear to be unique to that sequence and not seen in scrambled sequences. See Schwabe, C., New thoughts on the evolution of hormone-receptor systems, Comp.Biochem.Physiol.A 97, 101-106, 1990; Chaiken, I., Interactions and uses of antisense peptides in affinity technology, J.Chromatog. 597, 29-36, 1992; Labrou, N. and Clonis, Y.D., The affinity technology in downstream processing, J.Biotechnol. 36, 95-119, 1994; Root-Bernstein, R.S. and Holsworth, D.D., Antisense peptides: critical mini-review, J.Theoret.Biol. 190, 107-1199, 1998; Siemion, I.Z., Cebrat, M., and Kluczyk, A., The problem of amino acid complementarity and antisense peptides, Curr.Protein Pept.Sci. 5, 507-527, 2004.

2017 Version
Antisense Peptides
An antisense peptide is a peptide derived from the translation and transciption of the antisense strand of DNA; the transcription product of antisense RNA. 


The transciption of the antisense (non-coding, template) DNA strand yields the mRNA which codes for the sense peptide.  The mRNA is an exact replica (except for substitution of U for T) of the sense (coding, non-template) strand.  An antisense peptide is a peptide which would be coded for by a RNA molecule complementary to the mRNA coded by the antisense DNA strand.  This mRNA would be an exact replica(except for the substiution of U for T) of the sense DNA strand (Bost, K.L., Smith, E.M., and Blalock, J.E., Similarity between the coritcotropin (ACTH) receptor and a peptide encoded by an RNA that is complementary to ACTH mRNA, Proc.Natl.Acad.Sci. USA 82, 1372-375, 1985;  Rasmussen, U.B. and Hesch, R.D., On the antisense peptidews: the parathryroid hormone as an experimental example and a critical theoretical view, Biochem.Biophys.Res.Commun. 149, 930-938, 1987). This is hypothetical since it is not clear that this occurs in vivo although it not absolutely clear that this does not happen.  Below is shown the sense and antisense peptide for an exposed loop in IL-1β (Heal, J.R., Bino, S.,Roberts, G.W., Raynes, J.G., and Miller,A.D., Mechanistic investigation into complementary (antisense) peptide mini-receptor inhibitors of cytokine interleukin-1, ChemBioChem. 3, 76-85, 2002)

AA

Gln

Gly

Glu

Glu

Ser

Asn

Asp

 

5’

CAA

GGA

GGA

GAA

AGU

AAU

GAC

3’

3’

GUU

CCU

CUU

CUU

UCA

UUA

CUG

5’

AA

Leu

Ser

Phe

Phe

Thr

Ile

Val

 

 Some antisense peptides have been demonstrated to show affinity properties that appear to be unique to that sequence and not seen in scrambled sequences (Chaiken, I., Interactions and uses of antisense peptides in affinity technology, J.Chromatog. 597, 29-36, 1992 Root-Bernstein, R.S. and Holsworth, D.D., Antisense peptides: critical mini-review, J.Theoret.Biol. 190, 107-1199, 1998; Siemion, I.Z., Cebrat, M., and Kluczyk, A., The problem of amino acid complementarity and antisense peptides, Curr.Protein Pept.Sci. 5, 507-527, 2004; Miller, A.D., Sense-antisense (complementary) peptide interactions and the proteomic code; potential opportunities in biology and pharmaceutical science, Expert Opin.Biol.Ther. 15, 245-267, 2015).  The term complementary peptide has been used to describe antisense peptides as the concept is extended beyond a single product of the transcription of an antisense RNA ( Bhakoo, A., Raynes, J.G., Heal, J.R., Keller, M., and MIller, A.D., De-novo design of complementary (antisense) peptide mini-receptor inhibitor of interleukin 18 (IL-18), Mol.Immunol. 41, 1217-1224, 2004; Siemion, I.Z., Cebrat, M., and Khuczyk, A., The problem of amino acid complemtarity6 and antisense peptides, Curr.Prot.Pept.Sci. 5, 507-527, 2004).  Antisense/complementary peptides are of interest in autoimmujity (McGuire, K.L. and Holmes, D.S., Role of complementary proteins in autoimmuity: an old idea  re-emerges with new twists, Trends Immunol. 26, 367-372, 2005; Reynolds, J., Preston, G.A., Pressler, B.M., et al., Autoimmunity to the alpha 3 chain of type IV collagen in glomerulonephritis is triggered by ‘autoantigen complementarity’. J.Autoimmun. 59, 8-18, 2015).  It should be noted that the term antisense peptide overlaps with antisence peptide nucleic acid; the term antisense peptide nucleic acid refers to antisense oligonucleotide seequence on a peptde back composed of aminoethyl glycine (Nielsen, P.E., Egholm,M.,Berg, R.H., and Burchardt, O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide, Science 254, 1497-1500, 1991; NIelsen, P.E., Antisense peptide nucleic acids, Curr.Opin.Mol.Ther. 2,282-287, 2000). See also peptide nucleic acid, scrambled peptide, antisense peptide nucleic acids.